abstract：:An outbreak of an acute sporadic viral hepatitis occurring at three villages in the lower Shebli region of southern Somalia was studied. Sera were examined for antibodies to hepatitis E virus (HEV) antigen by a recently developed enzyme-linked immunosorbent assay. The prevalence of anti-HEV ranged from 77.8% to 94.0% ...

journal_title：Journal of medical virology

authors： Mushahwar IK,Dawson GJ,Bile KM,Magnius LO

更新日期：1993-07-01 00:00:00

abstract：:Female renal transplant recipients have an increased incidence of human papillomavirus (HPV) associated lesions, such as cervical dysplasia and neoplasia [Schneider et al., 1983]. In this study we tested the serological responses by enzyme-linked immunoadsorbent assays (ELISA) to 3 different antigenic regions of HPV t...

journal_title：Journal of medical virology

authors： Lewensohn-Fuchs I,Wester D,Bistoletti P,Elfgren K,Ohlman S,Dillner J,Dalianis T

更新日期：1993-07-01 00:00:00

abstract：:The prevalence of anti-HCV was studied in a South African area endemic for hepatitis B virus. A total of 35,685 volunteer blood donors (22,034 whites, 9,218 Asians, 3,077 Africans, 1,356 coloureds), 71 haemophiliacs, 84 chronic dialysis patients, 100 antenatal attenders, 212 nurses, and 20 HIV-positive male homosexual...

journal_title：Journal of medical virology

authors： Soni PN,Tait DR,Kenoyer DG,Fernandes-Costa F,Naicker S,Gopaul W,Simjee AE

更新日期：1993-05-01 00:00:00

abstract：:In order to assess the efficacy of interferon-alpha on hepatitis C viral RNA in patients with chronic hepatitis C, we analyzed the levels of HCV RNA in sera and liver tissues of 16 patients pre- and posttreatment using a semiquantitative polymerase chain reaction method. Fifteen of 16 patients were positive for anti-H...

journal_title：Journal of medical virology

authors： Liang TJ,Rustgi V,Galun E,Blum HE

更新日期：1993-05-01 00:00:00

abstract：:To evaluate the variation over time of seroreactivity to human papillomavirus type 11 (HPV-11) according to disease outcome, we selected a sample of 42 condyloma acuminatum patients from a group of subjects enrolled in a placebo-controlled trial of three alpha-interferon preparations administered parenterally for the ...

journal_title：Journal of medical virology

authors： Bonnez W,Da Rin C,Rose RC,Tyring SK,Reichman RC

更新日期：1993-04-01 00:00:00

abstract：:Virological studies were carried out on 3 to 36-month-old patients admitted to the Children's Hospital of the University of Modena with febrile syndrome from September 1990 to February 1991. Virological tests were carried out for human herpesvirus-6 (HHV-6), Epstein-Barr virus (EBV), cytomegalovirus (CMV), herpes simp...

journal_title：Journal of medical virology

authors： Portolani M,Cermelli C,Moroni A,Bertolani MF,Di Luca D,Cassai E,Sabbatini AM

更新日期：1993-02-01 00:00:00

abstract：:The ability of hepatitis A virus (HAV) to agglutinate human erythrocytes was used to develop IgM and IgG antibody capture haemadherence tests (MACHAT and GACHAT). Haemadherence was dependent on the pH of the red cell suspension and was best in the pH range 5.4 to 5.8. The tests were applied to serum, urine, and saliva...

journal_title：Journal of medical virology

authors： Perry KR,Parry JV

更新日期：1993-01-01 00:00:00

abstract：:The discovery of acyclovir marked the beginning of an exciting era in antiviral research. Early studies on the novel mode of action explained the selectivity of the compound and the remarkably narrow spectrum of activity against a subset of the herpesviruses. Throughout the past decade many clinical trials have demons...

journal_title：Journal of medical virology

authors： Darby G

更新日期：1993-01-01 00:00:00

abstract：:Genital herpes is a common, distressing infection whose incidence has been underestimated. Recent serological surveys, employing type-specific antibody assays, show a rising prevalence of previous herpes simplex virus (HSV) type 2 infections in post-adolescent populations in developed countries; many of these infectio...

journal_title：Journal of medical virology

authors： Kinghorn GR

更新日期：1993-01-01 00:00:00

abstract：:Varicella in the immunocompetent adult is an infrequent but potentially serious infection. Previous studies in immunocompetent hosts and normal adults have demonstrated the value of intravenous acyclovir in the treatment of varicella-zoster virus infections. Oral acyclovir has also shown efficacy in both normal adults...

journal_title：Journal of medical virology

authors： Wallace MR,Bowler WA,Oldfield EC 3rd

更新日期：1993-01-01 00:00:00

abstract：:A modified centrifugation culture technique and a polymerase chain reaction (PCR) is described for detection of early antigen and IE antigen DNA, respectively, for rapid and sensitive monitoring of active cytomegalovirus (HCMV) infection after organ transplantation. In a preliminary study, 541 clinical specimens (bloo...

journal_title：Journal of medical virology

authors： Prösch S,Kimel V,Dawydowa I,Krüger DH

更新日期：1992-12-01 00:00:00

abstract：:A procedure is described for the detection of hepatitis C virus (HCV) RNA in blood by means of the polymerase chain reaction (PCR) in which the reverse transcription step and two rounds of amplification are carried out in a single tube. This results in fewer manipulations, reduced risk of contamination, and economy of...

journal_title：Journal of medical virology

authors： Lin HJ,Shi N,Mizokami M,Hollinger FB

更新日期：1992-11-01 00:00:00

abstract：:A highly sensitive two-step polymerase chain reaction (PCR) method was evaluated for detection of human immunodeficiency virus type 1 (HIV-1) DNA in clinical specimens. The product resulting from the first amplification reaction is used as the template for the second PCR with an internal (nested) primer pair. Even whe...

journal_title：Journal of medical virology

authors： Zazzi M,Romano L,Brasini A,Valensin PE

更新日期：1992-11-01 00:00:00

abstract：:In order to assess the current seroepidemiology of hepatitis D virus (HDV) infection in Taiwan where hepatitis B virus (HBV) is hyperendemic, a total of 756 voluntary blood donors, 641 prostitutes, 1,014 patients with sexually transmitted diseases (STDs), and 628 drug abusers were studied. Radioimmunoassays were used ...

journal_title：Journal of medical virology

authors： Chen CJ,Tseng SF,Lu CF,Lin HC,You SL,Chen CS,Hwang SJ,Hsieh SF,Hsu ST

更新日期：1992-10-01 00:00:00

abstract：:Potential risk factors for the development of hepatocellular carcinoma were analysed in 40 Caucasian patients with this malignancy. A higher proportion (14 of 40; 35%) had evidence of hepatitis C virus (HCV) infection than had evidence of either hepatitis B virus (HBV) carriage (17.5%) or alcohol abuse (30%). In all 1...

journal_title：Journal of medical virology

authors： Garson JA,Wicki AN,Ring CJ,Joller H,Zala G,Schmid M,Buehler H

更新日期：1992-10-01 00:00:00

abstract：:One hundred twelve peripheral blood specimens were tested for the presence of cytomegalovirus (CMV) by the tube culture indirect immunoperoxidase (TC-IPA) procedure, the shell vial assay [shell vials were pre- and postinoculation treated with medium containing 2 of 10% fetal bovine serum (FBS) or 100 micrograms% corti...

journal_title：Journal of medical virology

authors： Lipson SM,Kaplan MH,Simon JK,Ciamician Z,Tseng LF

更新日期：1992-09-01 00:00:00

abstract：:The epidemiology of HTLV-I infection in female prostitutes was studied in a survey of 395 prostitutes from Callao, Peru (the port city of Lima), 72 prostitutes from Iquitos, Peru (another port city on the Amazon River), and 510 prenatal clinic patients from Lima. Prostitutes reported a mean of 8.8 years (range, 1-39 y...

journal_title：Journal of medical virology

authors： Wignall FS,Hyams KC,Phillips IA,Escamilla J,Tejada A,Li O,Lopez F,Chauca G,Sanchez S,Roberts CR

更新日期：1992-09-01 00:00:00

abstract：:Human herpesvirus 6 (HHV-6) prototype isolate GS is a newly identified lymphotropic herpesvirus and several subsequent herpes isolates were recognized as HHV-6 by their hybridization to a HHV-6(GS) DNA probe pZVH14. DNA restriction analysis and in vitro tropism studies show that HHV-6 isolates can be divided into two ...

journal_title：Journal of medical virology

authors： Chandran B,Tirawatnapong S,Pfeiffer B,Ablashi DV

更新日期：1992-08-01 00:00:00

abstract：:The effectiveness of a daily continuous infusion of interferon-alpha was evaluated in 12 patients (10 males, 2 females; mean age of 33 years, range 19-62) with biopsy-proven chronic active hepatitis C. Nine million units (MU) of recombinant interferon-alpha 2A (rIFN-alpha 2A) were administered by continuous subcutaneo...

journal_title：Journal of medical virology

authors： Carreño V,Tapia L,Ryff JC,Quiroga JA,Castillo I

更新日期：1992-07-01 00:00:00

abstract：:A quantitative polymerase chain reaction (PCR) assay for hepatitis C viral RNA (HCV-RNA) was used to monitor viraemia levels in six patients at multiple time points before, during, and after interferon therapy for chronic non-A, non-B hepatitis (NANBH). Prior to therapy, serum HCV-RNA was detected in all patients at a...

journal_title：Journal of medical virology

authors： Garson JA,Brillanti S,Ring C,Perini P,Miglioli M,Barbara L

更新日期：1992-07-01 00:00:00

abstract：:Attachment of hepatitis B virus to a hepatoblastoma cell line (HepG2) was examined using a synthetic peptide corresponding to the pre-S1 (21-47) region of the envelope protein. Scatchard analysis revealed a single class binding site of Kd 104 +/- 27 nM/l and 5.4 +/- 1.2 x 10(5) sites per cell. Competition of HBV with ...

journal_title：Journal of medical virology

authors： Dash S,Rao KV,Panda SK

更新日期：1992-06-01 00:00:00

abstract：:To determine risk factors responsible for primary cytomegalovirus (CMV) infection in Taiwan, samples of blood for antibody to CMV were obtained from 362 children aged 4 to 12 years: 58% were found to be positive for anti-CMV IgG antibody. Logistic regression analysis showed that seropositivity correlated with age, met...

journal_title：Journal of medical virology

authors： Shen CY,Chang WW,Chang SF,Chao MF,Huang ES,Wu CW

更新日期：1992-05-01 00:00:00

abstract：:Because in situ/filter hybridisation is not sensitive enough and because classical polymerase chain reaction (PCR) protocols are generally not sufficiently reproducible and specific, there is little accurate information on the prevalence of human papillomaviruses (HPV) 16, 18, and 33 infections in women without dyskar...

journal_title：Journal of medical virology

authors： Vandenvelde C,Scheen R,Van Pachterbeke C,Loriaux C,Decelle J,Hubert T,Delhaye C,Cattoor JP,Duys M,Van Beers D

更新日期：1992-04-01 00:00:00

abstract：:A hepatitis B vaccination campaign was carried out in a town of 60,000 inhabitants, Afragola, Campania, Italy, a hyperendemic area for hepatitis B where HBsAg prevalence was 13.4% and anti-HBc prevalence was 64.7%. This experimental pilot project aimed to reduce the incidence of both acute and asymptomatic viral hepat...

journal_title：Journal of medical virology

authors： Da Villa G,Piazza M,Iorio R,Picciotto L,Peluso P,De Luca G,Basile B

更新日期：1992-04-01 00:00:00

abstract：:Avidity maturation of IgG antibody responses directed against the structural proteins of rubella virus (E1, E2, and C) as well as whole rubella virus (RV) was assessed at sequential time intervals in 7 individuals following serologically confirmed wild rubella infection. Individual structural proteins were purified fr...

journal_title：Journal of medical virology

authors： Mauracher CA,Mitchell LA,Tingle AJ

更新日期：1992-03-01 00:00:00

abstract：:Hepatitis E virus (HEV), a positive-strand RNA agent, has been associated with enterically transmitted non-A, non-B hepatitis in Asia, Africa, and Mexico. To evaluate the role of HEV in an outbreak of hepatitis in Pakistan, we used immune electron microscopy to detect 1) antibody to HEV, for evidence of infection, and...

journal_title：Journal of medical virology

authors： Ticehurst J,Popkin TJ,Bryan JP,Innis BL,Duncan JF,Ahmed A,Iqbal M,Malik I,Kapikian AZ,Legters LJ

更新日期：1992-02-01 00:00:00

abstract：:Interaction between herpesviruses and human immunodeficiency virus (HIV)1 is postulated in the progression of HIV disease. In order to evaluate the specific antibody responses directed to Epstein-Barr virus (EBV) and cytomegalovirus (CMV) and to provide serological evidence suggesting reactivation of these viruses abl...

journal_title：Journal of medical virology

authors： Quesnel A,Pozzetto B,Touraine F,Moja P,Lucht F,De The G,Touraine JL,Gaudin O,Genin C

更新日期：1992-01-01 00:00:00

abstract：:Thirty-nine children without previous cytomegalovirus (CMV) infection received blood transfusion in the National Taiwan University Hospital. The overall transfusion-acquired CMV infection rate was 36% (14/39). Donor CMV seropositive rate was 70%. None of the nine children who had received seronegative blood became inf...

journal_title：Journal of medical virology

authors： Lee PI,Chang MH,Hwu WL,Kao CL,Lee CY

更新日期：1992-01-01 00:00:00

abstract：:The ability of a variety of epithelial, embryonal, placental, and neuronal cells to express the CD4 antigen and to be infected by human immunodeficiency virus 1 (HIV-1) was examined. Only two (IMR-32 and HeLa-T4) expressed CD4 detectable by indirect immunofluorescence, and both were infectable by HIV-1. Two others, a ...

journal_title：Journal of medical virology

authors： Morrison NK,McCarthy K,Hart CA

更新日期：1991-11-01 00:00:00

abstract：:Parainfluenza virus type 3 (Para 3) is an important childhood pathogen causing a significant amount of bronchiolitis and pneumonia in infants. Virus data were analysed over a twelve-year period (1978-1989), and a peak incidence of infection was observed in spring as in Houston, USA, unlike in the United Kingdom where ...

journal_title：Journal of medical virology

authors： de Silva LM,Cloonan MJ

更新日期：1991-09-01 00:00:00

abstract：:A solution of pooled cryo-precipitate for preparing factor VIII (F VIII) by the solvent/detergent method was contaminated experimentally with parvovirus B19-positive plasma to evaluate virus reduction achieved by the final steps of the F VIII production process. Virus reduction was at least 2 logs of the total amount ...

journal_title：Journal of medical virology

authors： Schwarz TF,Roggendorf M,Hottenträger B,Stolz W,Schwinn H

更新日期：1991-09-01 00:00:00

abstract：:The effect on cellular (c) oncogene RNA levels was investigated after infection of permissive cells with cell culture adapted strains (AD-169, C-87, Davis) and unadapted clinical isolates (82-1, 84-2, 85-1) of human cytomegalovirus (HCMV). The results indicate that both adapted and unadapted strains of HCMV induce sub...

journal_title：Journal of medical virology

authors： Boldogh I,AbuBakar S,Fons MP,Deng CZ,Albrecht T

更新日期：1991-08-01 00:00:00

abstract：:Physicochemical and molecular characterization studies of hepatitis C virus (HCV), the major causative agent of parenterally transmitted non-A, non-B hepatitis (PT-NANBH), strongly suggest that it is a pesti-/flavivirus-like virus. Additional studies show that the buoyant density of plasma-derived HCV in sucrose is si...

journal_title：Journal of medical virology

authors： Bradley D,McCaustland K,Krawczynski K,Spelbring J,Humphrey C,Cook EH

更新日期：1991-07-01 00:00:00

abstract：:Nineteen Chinese patients with chronic hepatitis B virus (HBV) infection, seropositive for HBV e antigen (HBeAg) and HBV DNA on at least three occasions in 6 months, were randomised to receive either recombinant human interferon-gamma (rIFN gamma) 0.1 mg/m2 intramuscularly thrice weekly for 16 weeks (n = 11) or no ant...

journal_title：Journal of medical virology

authors： Lau JY,Lai CL,Wu PC,Chung HT,Lok AS,Lin HJ

更新日期：1991-07-01 00:00:00

abstract：:A nationwide hepatitis B vaccination program was launched in Taiwan in 1984. To study the impact of this ongoing program on hepatitis B virus (HBV) infection, a follow-up seroepidemiologic study was carried out in 1989 in a Taipei district where pre-vaccination seroepidemiology had been studied. HBV markers were studi...

journal_title：Journal of medical virology

authors： Tsen YJ,Chang MH,Hsu HY,Lee CY,Sung JL,Chen DS

更新日期：1991-06-01 00:00:00

abstract：:The temporal distribution and clinical severity of rotavirus VP7 serotypes 1, 2, 3, and 4 recovered from 427 Venezuelan children with acute gastroenteritis over a period of 11 years were studied. Rotavirus VP7 serotype was established by ELISA serotyping in 298 (69.78%) of the specimens while the serotype of the remai...

journal_title：Journal of medical virology

authors： White L,García D,Boher Y,Blanco M,Pérez M,Romer H,Flores J,Pérez-Schael I

更新日期：1991-06-01 00:00:00

abstract：:Five HIV-1 isolates were obtained sequentially from a single seropositive individual during the later stages of AIDS. Four of these isolates were adapted to grow in a continuous human T-lymphocytic cell line. Comparative biological and biochemical studies of the virus isolates were made using persistently infected cul...

journal_title：Journal of medical virology

authors： Farrar GH,Roff MA,Amin T,Ball J,Parrett AM,Battacharyya U,Booth J,Wansbrough-Jones MH,Greenaway PJ

更新日期：1991-06-01 00:00:00

abstract：:The relationship of pre-bone-marrow transplant recipient and donor cytomegalovirus (CMV) antibody level to post-transplant recipient CMV excretion was investigated. CMV excretion was found to be related to pre-transplant recipient serological status, but not to level of antibody. This therefore contrasts with herpes s...

journal_title：Journal of medical virology

authors： Pillay D,Webster A,Lee D,Prentice HG,Griffiths PD

更新日期：1991-04-01 00:00:00

abstract：:In order to demonstrate the viral specificity of IgM-containing immune complexes (IgM-CIC) detected by a C1q assay in renal allograft recipients developing a CMV infection, a technique is described allowing: 1) the dissociation of IgM-CIC by action of an acid buffer, and 2) the characterization of the viral specificit...

journal_title：Journal of medical virology

authors： Basson J,Tardy JC,Aymard M

更新日期：1991-03-01 00:00:00

abstract：:An efficient method for cloning subgenomic fragments of the hepatitis B virus (HBV) was developed, utilizing its abundant single-stranded replicative intermediates. The total genomic DNA obtained from the liver tissue of patients with chronic HBV infection was treated by using the Klenow fragment of E. coli DNA polyme...

journal_title：Journal of medical virology

authors： Kajino K,Ohta Y,Miyamura T,Saito I

更新日期：1991-01-01 00:00:00